Abstract

Next-generation sequencing (NGS) has reformed infectious disease management, including COVID-19. While real-time polymerase chain reaction (PCR) is widely used for rapid pathogen detection, it requires predefined targets. NGS offers an unbiased approach, detecting multiple pathogens simultaneously without prior knowledge. Despite its potential, NGS implementation in clinical settings faces challenges like high costs and technical complexity. NGS platforms like Illumina, Ion Torrent, and Nanopore provide high-throughput sequencing, identifying pathogens and resistance markers. Applications include whole genome sequencing (WGS), metagenomic NGS (mNGS), and targeted NGS (tNGS). Integrating NGS with conventional methods could improve diagnostics, but current evidence is mixed for supporting its widespread clinical use.

Description

Copyright © 2024 The Author(s): This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted use, distribution, and reproduction in any medium for non-commercial use provided the original author and source are credited.

Publisher

South Asian Research Publication

Date of publication

Summer 6-20-2024

Language

english

Persistent identifier

http://hdl.handle.net/10950/4728

Document Type

Article

Publisher Citation

Rob E. Carpenter (2024). Advancing Clinical Microbiology: Applications and Future of Next-Generation Sequencing. SAR J Pathol Microbiol, 5(3), 107-111.

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