Document Type
Article
Publication Date
Spring 2-19-2014
Abstract
Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.
Persistant Identifier
http://hdl.handle.net/10950/4543
Publisher
CDC
Permanent Email Address
barbara.elliott@uthct.edu
Recommended Citation
Tettelin, Hervé; Davidson, Rebecca M.; Agrawal, Sonia; Aitken, Moira L.; Shallom, Shamira; Hasan, Nabeeh A.; Strong, Michael; Calado Nogueira de Moura, Vinicius; De Groote, Mary Ann; Duarte, Rafael S.; Hine, Erin; Parankush, Sushma; Su, Qi; Daugherty, Sean C.; Fraser, Claire M.; Brown-Elliott, Barbara A.; Wallace, Richard J. Jr.; Holland, Steven M.; Sampaio, Elizabeth P.; Olivier, Kenneth N.; Jackson, Mary; and Zelazny, Adrian M., "High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks" (2014). School of Medicine Faculty Publications and Presentations. Paper 5.
Description
This is a publication of the U.S. Government. The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.