Abstract

This dataset provides information from a study that validates a method for non-invasive urine collection utilizing sodium polyacrylate-based diapers, designed for polymerase chain reaction (PCR) detection of uropathogens. The dataset includes samples from 17 participants, comprising seven clinically contrived samples inoculated with known uropathogens and ten samples collected from volunteers wearing sodium polyacrylate-based diapers. The study involved optimizing urine extraction from the diaper matrices, ensuring minimal loss of diagnostic sensitivity for downstream quantitative PCR (qPCR) analysis. The qPCR targeted 22 uropathogens, six fungal species, and 18 antimicrobial resistance (AMR) genes, providing comprehensive molecular characterization of urinary pathogens. Comparative analyses were performed between diaper-derived and standard urine samples, with data demonstrating high concordance in detection outcomes, despite a modest average reduction in qPCR sensitivity (ΔCt of -1.65). Matrix effects were evaluated to determine the impact of the diaper material on qPCR amplification efficiency. The dataset is valuable for further research into non-invasive diagnostic techniques for urinary tract infections (UTIs), particularly for populations unable to provide midstream urine samples, such as infants and elderly individuals. Potential reuse of this dataset includes evaluating non-invasive urine collection efficacy, exploring the interaction between sample matrices and molecular diagnostics, and assessing the scalability of diaper-based sampling methods in diverse clinical settings.

Description

© 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/)

Publisher

Elsevier

Date of publication

Winter 10-30-2024

Language

english

Persistent identifier

http://hdl.handle.net/10950/4784

Document Type

Article

Publisher Citation

Vine T, Carpenter RE, Bridges D. Dataset for a Validated Method of Non-Invasive Urine Collection Using Sodium Polyacrylate-Based Diapers for PCR Detection of Uropathogens. Data in Brief. 2024 Oct 18:111036.

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