Abstract

Parkinson's’ disease (PD) is a neurodegenerative disorder characterized by the loss of midbrain dopaminergic (mDA) neurons that currently has no cure. It is primarily characterized in patients by motor symptoms that include but are not limited to: tremor at rest, rigidity, akinesia, and postural instability affecting the quality of life for over 8.5 million people worldwide. The neurotoxin 6-hydroxydopamine (6-OHDA) has been used to induce a Parkinson’s disease-like state in several animal models; however, assessment of 6-OHDA use in zebrafish demonstrated a lack of consensus on method and utility. We determined an optimized protocol utilizing 6-OHDA to produce a PD-like state as defined by a significant reduction of tyrosine hydroxylase (th). Additionally, we found the mechanism of action for 6-OHDA toxicity to be conserved as defined by the upregulation vii of super oxide dismutase sod1 and sod2, and down regulation of mitochondrial dysfunction indicator pink1.

Date of publication

Fall 12-2-2023

Document Type

Thesis

Language

english

Persistent identifier

http://hdl.handle.net/10950/4397

Committee members

Brent Bill, Ayman Hamouda, Ali Azghani, Matthew Greenwold

Degree

Masters in Biology

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