Document Type
Article
Publication Date
Spring 3-9-2024
Abstract
The continuing emergence of new strains of antibiotic-resistant bacteria has renewed interest in phage therapy, however, there has been limited progress in applying phage therapy to multi-drug resistant Mycobacterium tuberculosis (Mtb) infections. In this study, we show that bacteriophage strains D29 and DS6A can efficiently lyse Mtb H37Rv in 7H10 agar plates. However, only phage DS6A efficiently kills H37Rv in liquid culture and in Mtb-infected human primary macrophages. We further show in subsequent experiments that, after the humanized mice were infected with aerosolized H37Rv, then treated with DS6A intravenously, the DS6A treated mice showed increased body weight and improved pulmonary function relative to control mice. Furthermore, DS6A reduces Mtb load in mouse organs with greater efficacy in the spleen. These results demonstrate the feasibility of developing phage therapy as an effective therapeutic against Mtb infection. © The Author(s) 2024.
Persistant Identifier
http://hdl.handle.net/10950/5183
Publisher
Nature Research
Recommended Citation
Yang, Fan; Labani-Motlagh, Alireza; Bohorquez, Jose Alejandro; Moreira, Josimar Dornelas; and and additional authors, "Bacteriophage therapy for the treatment of Mycobacterium tuberculosis infections in humanized mice" (2024). School of Medicine Faculty Publications and Presentations. Paper 35.
Description
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