Abstract
This study investigates the impact of monoclonal antibody treatment on plasminogen activator inhibitor-1 (PAI-1) expression and secretion in transforming growth factor beta (TGFβ) activated pleural human mesothelial cells. In this study, we examined total PAI-1 levels in conditioned media from human pleural mesothelial cells under various conditions using immunoblot and enzyme-linked immunosorbent assay techniques (ELISA). Specifically, we targeted PAI-1 with monoclonal antibodies (mAbs) MA-33H1F7 and MA-8H9D4, which bind to unique epitopes on PAI-1. A reduction in PAI-1 levels was observed between control and antibody-treated cells by immunoblot analysis of cell conditioned media. ELISA results also revealed a reduction in total PAI-1 levels in the conditioned media of cells treated with antibodies. A decrease of 30-50% was observed upon addition of 50 µg/mL MA-33H1F7 and 50 µg/mL MA-8H9D4 of each antibody, with a similar reduction at 100 µg/mL concentration. Immunofluorescence (IFC) results corroborated the findings of the ELISA and Immunoblot, but quantification of the PAI-1 detected in IFC demonstrated a lack of statistical significance. This research underscores the complexity of PAI-1 regulation and highlights the need for further elucidation of the effects of antibody targeting on PAI-1 expression in TGFβ activated cells.
Date of publication
2024
Document Type
Thesis
Language
english
Persistent identifier
http://hdl.handle.net/10950/4715
Recommended Citation
Modukuri, Srikar, "Effect of Monoclonal Antibodies MA-33H1F7 and MA-8H9D4 on PAI-1 in Cells Activated with TGFβ" (2024). Biotechnology Theses. Paper 20.
http://hdl.handle.net/10950/4715
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